You are hereForums / Denitrification Genes / Updated Primer List - SH

Updated Primer List - SH

By sharg - Posted on 09 March 2009

Here is the updated (updated) list of primers for the 4 denitrification genes of interest. This list is very similar to Kurt's updated primer list and also works off of Ranae's primer list. The primer pairs highlighted in yellow are those that seem like the best first shot based on their use on soil samples, their reliability across studies, and if they have been used for SYBR green-based qPCR. There is no conclusive data to decide among the norB primer pairs (highlighted in orange) - the decision will depend on whether we look for cnorB, qnorB genes and/or use guild-specific sequences - thoughts? NB: There are multiple workbooks!

denitrification_qPCRprimer.xls36 KB

Secondary Links