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Protein quality control at the inner nuclear membrane

Nature - Wed, 12/17/2014 - 01:00

Protein quality control at the inner nuclear membrane

Nature 516, 7531 (2014). doi:10.1038/nature14096

Authors: Anton Khmelinskii, Ewa Blaszczak, Marina Pantazopoulou, Bernd Fischer, Deike J. Omnus, Gaëlle Le Dez, Audrey Brossard, Alexander Gunnarsson, Joseph D. Barry, Matthias Meurer, Daniel Kirrmaier, Charles Boone, Wolfgang Huber, Gwenaël Rabut, Per O. Ljungdahl & Michael Knop

The nuclear envelope is a double membrane that separates the nucleus from the cytoplasm. The inner nuclear membrane (INM) functions in essential nuclear processes including chromatin organization and regulation of gene expression. The outer nuclear membrane is continuous with the endoplasmic reticulum and is the site of membrane protein synthesis. Protein homeostasis in this compartment is ensured by endoplasmic-reticulum-associated protein degradation (ERAD) pathways that in yeast involve the integral membrane E3 ubiquitin ligases Hrd1 and Doa10 operating with the E2 ubiquitin-conjugating enzymes Ubc6 and Ubc7 (refs 2, 3). However, little is known about protein quality control at the INM. Here we describe a protein degradation pathway at the INM in yeast (Saccharomyces cerevisiae) mediated by the Asi complex consisting of the RING domain proteins Asi1 and Asi3 (ref. 4). We report that the Asi complex functions together with the ubiquitin-conjugating enzymes Ubc6 and Ubc7 to degrade soluble and integral membrane proteins. Genetic evidence suggests that the Asi ubiquitin ligase defines a pathway distinct from, but complementary to, ERAD. Using unbiased screening with a novel genome-wide yeast library based on a tandem fluorescent protein timer, we identify more than 50 substrates of the Asi, Hrd1 and Doa10 E3 ubiquitin ligases. We show that the Asi ubiquitin ligase is involved in degradation of mislocalized integral membrane proteins, thus acting to maintain and safeguard the identity of the INM.

Categories: Literature

Corrigendum: Activation and repression by oncogenic MYC shape tumour-specific gene expression profiles

Nature - Wed, 12/17/2014 - 01:00

Corrigendum: Activation and repression by oncogenic MYC shape tumour-specific gene expression profiles

Nature 516, 7531 (2014). doi:10.1038/nature14054

Authors: Susanne Walz, Francesca Lorenzin, Jennifer Morton, Katrin E. Wiese, Björn von Eyss, Steffi Herold, Lukas Rycak, Hélène Dumay-Odelot, Saadia Karim, Marek Bartkuhn, Frederik Roels, Torsten Wüstefeld, Matthias Fischer, Martin Teichmann, Lars Zender, Chia-Lin Wei, Owen Sansom, Elmar Wolf & Martin Eilers

Nature511, 483–487 (2014); doi:10.1038/nature13473In this Letter, the ArrayExpress microarray data set accession number was wrongly given as E-MTAB-1524; the correct accession number is E-MTAB-1886. This has been corrected in the online versions of the paper.

Categories: Literature

Falling Gasoline Prices Have Little Effect on Car Travel, Analysis Shows

Yale Environment 360 - Tue, 12/16/2014 - 11:54

Although the average retail price of gasoline in the U.S. has fallen 28 percent from its peak in June 2014,

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Gas prices vs. miles driven the decline may not have much effect on automobile travel and gasoline consumption, according to an analysis by the U.S. Energy Information Administration (E.I.A.). Typically, an increase in the price of a product leads to lower demand, and vice versa — a concept known as price elasticity. Air travel, for example, tends to be highly elastic: A 10-percent increase in the price of air fares leads to an even greater decrease in air travel. Automobile travel tends to be much less elastic, however. According to E.I.A. data, it takes a 25- to 50-percent decrease in the price of gasoline to increase automobile travel by just 1 percent. One reason for this is that the distance people drive to work and for daily errands is relatively fixed, analysts say. Increased vehicle fuel economy also balances out increases in miles traveled, leading to more stability in gasoline consumption.

Categories: Environmental News

Keep asking the question

Nature - Tue, 12/16/2014 - 01:00

Keep asking the question

Nature 516, 7531 (2014). doi:10.1038/516287a

Scientists must push to preserve a small part of a large US survey that provides essential information on the ever-changing scientific workforce.

Categories: Literature

Honest brokers

Nature - Tue, 12/16/2014 - 01:00

Honest brokers

Nature 516, 7531 (2014). doi:10.1038/516288a

Climate negotiations in Lima stumbled on transparency, but there is time to adjust.

Categories: Literature

Putin’s Russia divides and enrages scientists

Nature - Tue, 12/16/2014 - 01:00

Putin’s Russia divides and enrages scientists

Nature 516, 7531 (2014). http://www.nature.com/doifinder/10.1038/516298a

Author: Quirin Schiermeier

Are geopolitical tensions destroying important links with the West, or can Russian research go it alone?

Categories: Literature

Scientific method: Defend the integrity of physics

Nature - Tue, 12/16/2014 - 01:00

Scientific method: Defend the integrity of physics

Nature 516, 7531 (2014). doi:10.1038/516321a

Authors: George Ellis & Joe Silk

Attempts to exempt speculative theories of the Universe from experimental verification undermine science, argue George Ellis and Joe Silk.

Categories: Literature

Correction

Nature - Tue, 12/16/2014 - 01:00

Correction

Nature 516, 7531 (2014). http://www.nature.com/doifinder/10.1038/516299a

The News Feature ‘Ebola’s lost ward’ (Nature513, 474–477; 2014) incorrectly stated that nurse Veronica Koroma contracted Ebola.

Categories: Literature

Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice

ISME - Tue, 12/16/2014 - 01:00

Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice

The ISME Journal 9, 1 (January 2015). doi:10.1038/ismej.2014.99

Authors: Jingjing Wang, Huang Tang, Chenhong Zhang, Yufeng Zhao, Muriel Derrien, Emilie Rocher, Johan ET van-Hylckama Vlieg, Katherine Strissel, Liping Zhao, Martin Obin & Jian Shen

Categories: Literature

From community approaches to single-cell genomics: the discovery of ubiquitous hyperhalophilic Bacteroidetes generalists

ISME - Tue, 12/16/2014 - 01:00

From community approaches to single-cell genomics: the discovery of ubiquitous hyperhalophilic Bacteroidetes generalists

The ISME Journal 9, 16 (January 2015). doi:10.1038/ismej.2014.95

Authors: María Gomariz, Manuel Martínez-García, Fernando Santos, Francisco Rodriguez, Salvador Capella-Gutiérrez, Toni Gabaldón, Ramon Rosselló-Móra, Inmaculada Meseguer & Josefa Antón

Categories: Literature

Growth form defines physiological photoprotective capacity in intertidal benthic diatoms

ISME - Tue, 12/16/2014 - 01:00

Growth form defines physiological photoprotective capacity in intertidal benthic diatoms

The ISME Journal 9, 32 (January 2015). doi:10.1038/ismej.2014.105

Authors: Alexandre Barnett, Vona Méléder, Lander Blommaert, Bernard Lepetit, Pierre Gaudin, Wim Vyverman, Koen Sabbe, Christine Dupuy & Johann Lavaud

Categories: Literature

Early colonizing Escherichia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state

ISME - Tue, 12/16/2014 - 01:00

Early colonizing Escherichia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state

The ISME Journal 9, 46 (January 2015). doi:10.1038/ismej.2014.111

Authors: Julie Tomas, Julie Reygner, Camille Mayeur, Robert Ducroc, Stephan Bouet, Chantal Bridonneau, Jean-Baptiste Cavin, Muriel Thomas, Philippe Langella & Claire Cherbuy

Categories: Literature

Water fleas require microbiota for survival, growth and reproduction

ISME - Tue, 12/16/2014 - 01:00

Water fleas require microbiota for survival, growth and reproduction

The ISME Journal 9, 59 (January 2015). doi:10.1038/ismej.2014.116

Authors: Marilou P Sison-Mangus, Alexandra A Mushegian & Dieter Ebert

Categories: Literature

Interpreting 16S metagenomic data without clustering to achieve sub-OTU resolution

ISME - Tue, 12/16/2014 - 01:00

Interpreting 16S metagenomic data without clustering to achieve sub-OTU resolution

The ISME Journal 9, 68 (January 2015). doi:10.1038/ismej.2014.117

Authors: Mikhail Tikhonov, Robert W Leach & Ned S Wingreen

Categories: Literature

High prevalence of biofilm synergy among bacterial soil isolates in cocultures indicates bacterial interspecific cooperation

ISME - Tue, 12/16/2014 - 01:00

High prevalence of biofilm synergy among bacterial soil isolates in cocultures indicates bacterial interspecific cooperation

The ISME Journal 9, 81 (January 2015). doi:10.1038/ismej.2014.96

Authors: Dawei Ren, Jonas S Madsen, Søren J Sørensen & Mette Burmølle

Categories: Literature

A single genus in the gut microbiome reflects host preference and specificity

ISME - Tue, 12/16/2014 - 01:00

A single genus in the gut microbiome reflects host preference and specificity

The ISME Journal 9, 90 (January 2015). doi:10.1038/ismej.2014.97

Authors: A Murat Eren, Mitchell L Sogin, Hilary G Morrison, Joseph H Vineis, Jenny C Fisher, Ryan J Newton & Sandra L McLellan

Categories: Literature

Life on the edge: functional genomic response of Ignicoccus hospitalis to the presence of Nanoarchaeum equitans

ISME - Tue, 12/16/2014 - 01:00

Life on the edge: functional genomic response of Ignicoccus hospitalis to the presence of Nanoarchaeum equitans

The ISME Journal 9, 101 (January 2015). doi:10.1038/ismej.2014.112

Authors: Richard J Giannone, Louie L Wurch, Thomas Heimerl, Stanton Martin, Zamin Yang, Harald Huber, Reinhard Rachel, Robert L Hettich & Mircea Podar

Categories: Literature

Quorum sensing enhancement of the stress response promotes resistance to quorum quenching and prevents social cheating

ISME - Tue, 12/16/2014 - 01:00

Quorum sensing enhancement of the stress response promotes resistance to quorum quenching and prevents social cheating

The ISME Journal 9, 115 (January 2015). doi:10.1038/ismej.2014.98

Authors: Rodolfo García-Contreras, Leslie Nuñez-López, Ricardo Jasso-Chávez, Brian W Kwan, Javier A Belmont, Adrián Rangel-Vega, Toshinari Maeda & Thomas K Wood

Categories: Literature

The ambrosia symbiosis is specific in some species and promiscuous in others: evidence from community pyrosequencing

ISME - Tue, 12/16/2014 - 01:00

The ambrosia symbiosis is specific in some species and promiscuous in others: evidence from community pyrosequencing

The ISME Journal 9, 126 (January 2015). doi:10.1038/ismej.2014.115

Authors: Martin Kostovcik, Craig C Bateman, Miroslav Kolarik, Lukasz L Stelinski, Bjarte H Jordal & Jiri Hulcr

Categories: Literature

Adhesion as a weapon in microbial competition

ISME - Tue, 12/16/2014 - 01:00

Adhesion as a weapon in microbial competition

The ISME Journal 9, 139 (January 2015). doi:10.1038/ismej.2014.174

Authors: Jonas Schluter, Carey D Nadell, Bonnie L Bassler & Kevin R Foster

Categories: Literature

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